Single-nucleotide polymorphism discovery by targeted DNA photocleavage
نویسندگان
چکیده
منابع مشابه
Single-nucleotide polymorphism discovery by targeted DNA photocleavage.
Single-nucleotide polymorphisms are the largest source of genetic variation in humans. We report a method for the discovery of single-nucleotide polymorphisms within genomic DNA. Pooled genomic samples are amplified, denatured, and annealed to generate mismatches at polymorphic DNA sites. Upon photoactivation, these DNA mismatches are then cleaved site-specifically by using a small molecular pr...
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Single nucleotide polymorphisms (SNPs) have rarely been exploited in nonhuman and nonmodel organism genetic studies. This is due partly to difficulties in finding SNPs in species where little DNA sequence data exist, as well as to a lack of robust and inexpensive genotyping methods. We have explored one SNP discovery method for molecular ecology, evolution, and conservation studies to evaluate ...
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In the recent past, several publications have appeared in Drug Metabolism and Disposition that described the results of single nucleotide polymorphism (SNP) discovery projects. The publications were limited to only this aspect of the science and failed to include any studies that extended beyond the discovery phase. No attempt was made to elucidate the possible functional significance of the id...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2004
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0406169101